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Osteogenic differentiation of human mesenchymal stem cells induced by nanostructures
Author(s) -
CHOI SEON YOUNG,
RYU PAN DONG,
JOO SANGWOO,
LEE SO YEONG
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1146.11
Subject(s) - mesenchymal stem cell , chemistry , alizarin red , microbiology and biotechnology , colloidal gold , cellular differentiation , alkaline phosphatase , staining , nanotechnology , nanoparticle , biochemistry , materials science , biology , medicine , pathology , enzyme , gene
In our previous study, chitosan‐conjugated gold nanoparticles altered expression of miRNAs related to bone and cartilage formation. Several studies suggest that many types of nanoparticles induce differentiation of human mesenchymal stem cells (hMSCs) into determined lineage. We hypothesized that various nanostructures promote osteogenic differentiation of hMSCs. In order to examine promoted osteogenic differentiation of hMSCs, PEGylated graphene oxide, gold nanorods and chitosan‐conjugated gold nanoparticles were added to an osteogenic induction medium for 7 and 14 days. For the study, alizarin red staining, alamar blue assay and quantitative real time PCR were performed. Our results demonstrate that nanostructures promote the deposit of calcium content in hMSCs at non‐toxic concentrations. Also, the expression of marker genes related to the early stage of osteogenic differentiation was increased. These results indicate that nanostructures may be used as a promoting reagent for bone formation. This study received support from the National Research Foundation of Korea (2012R1A2A2A01047151).