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Lysophosphatidic acid induces reactive oxygen species generation through PLC/PKC/Nox Pathway in PC‐3 prostate cancer cells
Author(s) -
Lin ChuCheng,
Lin ChuanEn,
Lin YuehChien,
Lee Hsinyu
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1144.5
Subject(s) - lysophosphatidic acid , reactive oxygen species , nadph oxidase , transfection , chemistry , cancer research , small interfering rna , cancer cell , microbiology and biotechnology , cancer , biology , medicine , receptor , biochemistry , gene
Prostate cancer is one of the most frequently diagnosed cancers in males. PC‐3 is a popular cell model for investigating late prostate cancer behaviors. Lysophosphatidic acid (LPA) is a lysophospholipid that mediates several cell behaviors in cancers such as proliferation, migration and adhesion. In our previous study, LPA enhances VEGF‐C expression in PC‐3 cells through activating the generation of reactive oxygen species (ROS), which is known as an important secondary messenger in cancer progression. By using flow cytometry, we found that LPA triggers ROS generation within ten minutes and ROS production can be suppressed by antioxidant, N‐acetylcysteine. In addition, LPA 1/3 antagonist Ki16425 treatment and siRNA transfection efficiently suppressed the LPA induced ROS production that indicates LPA 1 and LPA 3 are involved in this pathway. As downstream signals of LPA, PLC and PKC were also suggested to participate in LPA induced ROS generation by using specific inhibitors and siRNAs. Furthermore, NADPH oxidase inhibitor DPI can block this ROS generation pathway. Overall, we demonstrated that LPA induces ROS generation through PLC/PKC/Nox pathway in PC‐3 prostate cancer cells.