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N‐acetylcysteine administration modulates NADPH oxidase in skeletal muscle of rats with heart failure
Author(s) -
Martinez Paula F,
Fernandes D C,
Bonomo C,
Cezar M D M,
Oliveira S A,
Zornoff L A M,
Okoshi K,
Laurindo F R,
Okoshi M P
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1143.9
Subject(s) - nadph oxidase , acetylcysteine , myocardial infarction , soleus muscle , medicine , skeletal muscle , heart failure , endocrinology , antioxidant , oxidase test , oxidative stress , pharmacology , chemistry , biochemistry , enzyme
This study aimed to evaluate the influence of antioxidant N‐ acetylcysteine on NADPH oxidase (NOX) activity and gene expression in soleus muscle of rats with myocardial infarctioninduced heart failure. Methods Myocardial infarction (MI) was induced by left coronary occlusion. Four months later, rats were assigned to three groups: Sham (n=8), MI‐C (MI without treatment, n=8), and MI‐NAC (MI treated with N ‐acetylcysteine, 120 mg/kg/day, n=8). Rats with small MI were excluded. Six months after surgery, NOX activity was assessed by HPLC analysis of dihydroethidium oxidation fluorescent products in soleus muscle. Gene expression of NOX complex subunits was evaluated by RT‐PCR. Results MI size did not differ between MI‐C and MI‐NAC groups (p>;0.05, Student's t test). NADPH oxidase activity was not different between MI‐C and Sham, but it was reduced in MI‐NAC compared to MI‐C group. Table shows the RT‐PCR results (ANOVA and Bonferroni; *p<0.05 vs. Sham, #p<0.05 vs. MI‐C): Conclusion N ‐acetylcysteine administration reduces NADPH oxidase activity and gene expression in soleus muscle during heart failure. Support: FAPESP and CNPq.