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Elevated peripheral blood mononuclear cell‐derived superoxide production in healthy young black men
Author(s) -
Deo Shekhar H,
Holwerda Seth W,
Keller David M,
Fadel Paul J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1142.1
Subject(s) - superoxide , nadph oxidase , peripheral blood mononuclear cell , oxidative stress , reactive oxygen species , endocrinology , medicine , superoxide dismutase , intracellular , western blot , chemistry , andrology , microbiology and biotechnology , biology , enzyme , biochemistry , gene , in vitro
Several studies have demonstrated that blacks exhibit elevations in plasma oxidative stress. However, the sources and mechanisms contributing to the elevation in oxidative stress remain unclear. Given recent work indicating that peripheral blood mononuclear cells (PBMCs) can be a major source of angiotensin II type 1 receptor (AT 1 R)‐NADPH oxidase derived superoxide production, we sought to examine this pathway in mediating the increase in systemic oxidative stress in black men. PBMCs were isolated from whole blood in 8 black and 8 age‐matched white men. Intracellular superoxide production was measured using dihydroethidium fluorescence and protein expression of AT 1 R and NADPH oxidase subunit, gp91 phox were assessed using western blot. Black men showed elevated resting intracellular superoxide production (7.5±1.7 blacks vs. 3.1±1.1 whites, RFU; P <0.05) as well as protein expression for AT 1 R and gp91 phox ( P <0.05 vs. whites). Furthermore, for all subjects, AT 1 R expression was related to gp91 phox (R = 0.67; P <0.05). These preliminary findings suggest that young black men exhibit greater resting PBMC derived superoxide production potentially via an up‐regulation of the AT 1 R‐NADPH oxidase pathway. Supported by NIH Grant R15HL096103 (DMK) and RO1 HL093167 (PJF)