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Arterial Stiffness in Diet‐induced Obese Mice is Reversed by Weight Loss
Author(s) -
Shiang Tina,
Al Sayah Leona,
Fry Jessica,
Weisbrod Robert,
Bajpai Saumendra,
ReinhartKing Cynthia,
Cohen Richard,
Seta Francesca
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1138.3
Subject(s) - arterial stiffness , medicine , pulse wave velocity , endocrinology , blood pressure , hyperinsulinemia , insulin resistance , diastole , weight loss , metabolic syndrome , obesity , cardiology
BACKGROUND We previously showed that C57Bl/6J male mice fed a diet rich in fat (35%) and sucrose (16.5%) (HFHS) develop obesity, glucose intolerance, insulin resistance, chronic inflammation, cardiac diastolic and kidney dysfunctions compared to normal diet (ND). We also showed that HFHS‐fed mice develop arterial stiffness (AS), measured in vivo by pulse wave velocity (PWV), within 2 mos and systolic hypertension, measured by radiotelemetry, after 6 mos compared to ND. AIM We sought to test the hypothesis that AS and hypertension would normalize in obese mice after reversal to ND. METHODS AND RESULTS Mice fed HFHS for 5 mos were fed ND for 2.5 mos with bi‐weekly assessments of metabolic and cardiovascular parameters. Diet reversal induced a severe weight loss in obese mice with a 2‐fold reduction in fat mass and hyperinsulinemia and no change in lean mass. PWV was significantly reduced from 5.3 ± 0.7 to 3.37 ± 0.4 m/sec after 1.5 mo reversal to ND. Similarly, systolic blood pressure was significantly decreased at this time point (from 135.7 ± 3.8 to 124.2 ± 2.7 mmHg). Atomic force on aortic rings, an in vitro indicator of structural stiffness of the intima, was increased after HFHS and significantly decreased after 4 mos reversal to ND. CONCLUSIONS Obesity‐induced arterial stiffness and hypertension are reversible by fat loss with normalization of metabolic state. NHLBI grant HL105287–01.