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Regulation of antioxidant enzyme expression in response to exercise in skeletal muscles of dyslipidemic mice
Author(s) -
Nguyen Albert Quyen,
Thorin Eric
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1136.14
Subject(s) - dyslipidemia , medicine , endocrinology , gpx1 , antioxidant , myograph , endogeny , chemistry , endothelium , glutathione peroxidase , oxidative stress , obesity , biochemistry , catalase
Dyslipidemia combined with a sedentary lifestyle is a well‐characterized combination of risk factor for cardiovascular diseases (CVD). Although preventive physical exercise delays the onset of CVD, the underlying mechanisms are not clear. We hypothesized that exercise regulates antioxidant enzyme expression that counteracts the deleterious effects of dyslipidemia. To test this hypothesis, 3‐month old (m/o) severely dyslipidemic (LDLR−/−, hApoB+/+) mice (SD) and wild type (WT) controls were exposed or not (sedentarity, SED) for 3 months to voluntary physical training (PT, □4.5 km/day). At 6‐m/o, the endothelial function of the femoral artery was measured using a pressure myograph and the surrounding skeletal muscles were isolated to quantify antioxidant gene expression by qPCR. No differences in endothelial function were noted between groups. In contrast, GPx1 expression decreased by 25% (p<0.05) in SD mice; PT, however, increased (p<0.05) its expression in both WT (+25%) and SD (+54%) mice. Prdx3 mRNA was higher (p<0.05) in SD compared to WT by 44% and 50% in SED and PT mice. Likewise, MnSOD and Txn1 expressions were elevated in SD mice compared to the other groups (p<0.05) and that was prevented by PT. In conclusion, analysis of mRNA expression reveals that the regulation of antioxidant enzymes is sensitive to dyslipidemia and PT, unveiling the dynamic and sensitive nature of the endogenous antioxidants.