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Endogenous hydrogen sulfide in the rostral ventrolateral medulla (RVLM) mediates ventilatory response induced by hypoxia.
Author(s) -
Donatti Alberto Ferreira,
Kwiatkoski Marcelo,
Soriano Renato Nery,
Carnio Evelin Capelari,
Branco Luiz Guilherme Siqueira
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1135.12
Subject(s) - microinjection , hypoxia (environmental) , chemistry , rostral ventrolateral medulla , endocrinology , hydrogen sulfide , medicine , ventilation (architecture) , hypoxic ventilatory response , cannula , respiration , respiratory system , endogeny , anesthesia , biochemistry , oxygen , medulla oblongata , anatomy , central nervous system , surgery , sulfur , mechanical engineering , organic chemistry , engineering
We investigated the involvement of hydrogen sulfide (H 2 S), in the RVLM, in the ventilator response of hypoxia. Wistar rats were implanted with a stainless steel guide cannula above the RVLM region. The animals were microinjected with a CBS inhibitor (amino‐oxyacetate ‐ AOA) in two different concentration, 20 fmol/2 μl and 200 fmol/2 μl or phosphate buffer (PH 7.4) (2 μl) as vehicle. After the microinjection, ventilation of the animals was measured during 60 min in normoxia or hypoxia. During normoxia, microinjection of AOA did not change ventilation compared with PBS (p>;0.05). When microinjected with AOA 20 fmol/2 μl and exposed to hypoxia, the rats showed a significant increase (p<0.05) in ventilation (1506±169 ml/kg/min) compared with PBS (1279.78±90 ml/kg/min). When microinjected AOA 200 fmol/2 μl and exposed to hypoxia, the rats showed an increased (p<0.05) frequency (143.4±7.7 breaths/min) compared with PBS (114.9±3.8 breaths/min). The present results show that endogenous H 2 S plays an important role in modulating ventilatory response to hypoxia in the RVLM. Financial support: FAPESP, CAPES, CNPq (Brazil).