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Muscle perilipin 3 is reduced using in vitro and in vivo exercise models and negatively associated with exercise lipid oxidation
Author(s) -
Covington Jeffrey D,
Galgani Jose E.,
Rustan Arild C,
Zhang Zhengyu,
Moro Cedric,
Smith Steven R,
Ravussin Eric,
Bajpeyi Sudip
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1132.5
Subject(s) - perilipin , medicine , in vivo , endocrinology , lipid droplet , chemistry , lipid oxidation , lipid metabolism , ex vivo , vastus lateralis muscle , in vitro , lipolysis , skeletal muscle , biochemistry , biology , adipose tissue , antioxidant , microbiology and biotechnology
Perilipin 2 (PLIN2) and perilipin 3 (PLIN3) have been shown to play important roles in the regulation of lipid droplet dynamics. Our aim was to understand changes in PLIN2 and PLIN3 protein content with increased lipid utilization during both in vitro and in vivo models of exercise. Pharmacological treatment (30μM palmitate, 4μM forskolin, 0.5μM ionomycin), which mimics exercise in human myotubes in vitro , showed that PLIN2 protein was unaltered while PLIN3 was reduced (p=0.01). Likewise, using biopsies from the vastus lateralis before and after a long endurance exercise bout (50% VO 2Max until 650 kcal energy expenditure obtained) in 20 healthy, young males (age: 24.0 ± 4.5yrs, BMI: 23.6 ± 1.8 kg/m 2 ), we found no change in PLIN2 and a reduction in PLIN3 (p=0.03). Moreover, the change in PLIN3 during exercise was positively correlated with cumulative whole‐body lipid oxidation measured by indirect calorimetry (r = 0.53; p = 0.04), changes in ex vivo muscle palmitate oxidation (r = 0.63, p = 0.01), and negatively correlated with changes in intramyocelluar lipid (r = −0.47; p = 0.05). These data show that both in vitro and in vivo models of exercise do not change PLIN2 but decrease PLIN3 expression. The reduction in PLIN3 is associated with decreased whole body as well as muscle lipid oxidation.