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Determination of exercise modality employing serum metabolomics profiling in type 2 diabetes: relation to clinical outcomes
Author(s) -
Yang Jaeun Jane,
Palmnas Marie,
Su Juliet,
Kirtadi Lawrence,
Vogel Hans,
Sigal Ron,
Shearer Jane
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1132.4
Subject(s) - metabolomics , aerobic exercise , type 2 diabetes , insulin resistance , medicine , diabetes mellitus , chemistry , endocrinology , chromatography
It is generally acknowledged that exercise training, both aerobic and resistance, is conducive to health and decreases chronic disease risk. However, little information exists on type of exercise that confers the greatest benefit. The purpose of this study is to use metabolomics strategy to i) interrogate the effects of exercise training modality on serum biochemistry and ii) compare changes in metabolomic profiles with clinical data (i.e. weight, BMI, HbA1c) to determine any correlation. Individuals with type 2 diabetes (n=40) were recruited as a part of the Diabetes Aerobic Resistance Exercise (DARE) study. After a 4‐week run‐in program, individuals were randomly assigned into one of four groups: control, aerobic, resistance or a combination of aerobic + resistance training for 22wk. Serum was collected at baseline and at 6mo. Serum samples were analyzed for metabolites using gas‐chromatography‐ mass spectrometry (GC‐MS) and Proton Nuclear Magnetic Resonance Spectroscopy (H1‐NMR). Changes in metabolism involved metabolites such as amino acids, organic acid, and carbohydrates. Aerobic exercise elevated the rate of tricarboxylic acid cycle and antioxidant activity, while resistance exercise lead to hypertrophy and urea markers. In summary, GC‐MS and NMR based metabolomics analysis proved to effectively reveal exercise modality and explain differential clinical outcomes between treatment groups.