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Effects of combined aerobic and resistance exercise training on apoptosis and autophagy of CD4+ lymphocytes in sedentary men
Author(s) -
Lin YiHuei,
Wang JongShyan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1132.13
Subject(s) - aerobic exercise , apoptosis , autophagy , lymphocyte , medicine , endocrinology , resistance training , immunology , chemistry , biochemistry
Physical exercise may influence CD4 + lymphocyte activity and potentially modulate adaptive immune function. This study investigated how combined aerobic and resistance exercise training influenced apoptosis and autophagy of CD4 + lymphocytes. Twelve healthy sedentary males were trained on a bicycle ergometer (60%VO 2max for 30 minutes) for 3 days/week and an isokinetic dynamometer (alternating 60°–180°/s for 6 to 10 sessions) for 2 days/week for 6 weeks. Apoptotic and autophagic responses in CD4 + lymphocyte were determined by monitoring corresponding biological markers. The experimental results demonstrated that strenuous, acute exercise simultaneously lost mitochondrial membrane potential, activated caspase‐3, and up‐regulated LAMP‐2, which were accompanied by the increases in exposure of phosphatidylserine (PS) and staining of acridin orange (AO) in CD4 + lymphocytes. However, combined aerobic and resistance exercise training decreased CD4 + lymphocyte LAMP‐2 content, AO staining, and PS exposure at rest status. Moreover, this training regimen also suppressed the extent of increased PS exposure by intense exercise. Therefore, we conclude that strenuous, acute exercise facilitates CD4 + lymphocyte autophagy and apoptosis, whereas combined aerobic and resistance exercise training attenuates apoptotic activation of CD4 + lymphocyte induced by vigorous exercise.