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Basal expression of VEGF does not correlate with loss of skeletal muscle capillarity with detraining
Author(s) -
Olenich Sara,
Roberts Kathleen,
Audet Gerald,
Olfert I. Mark
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1132.10
Subject(s) - nucleolin , basal (medicine) , endocrinology , medicine , angiogenesis , vascular endothelial growth factor , skeletal muscle , endostatin , matrix metalloproteinase , vegf receptors , chemistry , cytoplasm , biochemistry , insulin , nucleolus
Temporal expression of positive and negative angiogenic factors in response to training and detraining is still poorly described. Protein expression of anti‐angiogenic thrombospondin‐1 (TSP‐1) and endostatin as well as pro‐angiogenic vascular endothelial growth factor (VEGF), matrix metalloproteinases ‐2, ‐9 (MMP‐2, MMP‐ 9), and nucleolin (a nuclear protein involved with synthesis and maturation of ribosomes) were examined after training and detraining in mouse gastrocnemius muscle. Mice were placed in one of 5 groups (n=12/group): control (did not run), trained (voluntary wheel ran 21 days), or detrained (21 day trained, then detrained) for 7, 14, or 28 days. When compared to control mice, training increased capillary‐to‐fiber ratio (C:F) and resulted in an increase of basal VEGF and MMP‐9 ( p <0.05), all other factors remained unchanged. After 7 days of detraining, C:F returned to control levels, basal VEGF remained elevated and TSP‐1 increased ( p <0.05). Following 14 days of detraining, basal VEGF levels were still elevated, TSP‐1 fell to baseline, and nucleolin declined compared to controls ( p <0.05). After 28 days of detraining, basal VEGF returned to baseline, TSP‐1 remained unchanged while endostatin and MMP‐2 levels decreased compared to controls ( p <0.05). These data suggest that an increase in TSP‐1 and a decrease in MMP's and nucleolin, despite elevated VEGF, correlate with detraining losses of skeletal muscle capillarity. Supported by NIH 5P20RR016477; NIH 5T32HL090610; AHA 10BGIA3630002