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Reactive oxygen species in saliva related acute myocardial infarction
Author(s) -
Rubio Maria C,
Puntarulo Susana,
Gonzalez Paula,
Ramos Cecilia,
Lewin Pablo,
Lifshitz Fima,
Friedman Silvia,
Nicolosi Liliana
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1130.6
Subject(s) - saliva , myocardial infarction , reactive oxygen species , oxidative stress , medicine , chest pain , biomarker , troponin , cardiology , chemistry , biochemistry
Acute myocardial infarction (AMI) is a highly dynamic event, associated with increasing production of reactive oxygen species (ROS). The imbalance between ROS production and antioxidative defenses, leads to a condition known as oxidative stress that may increase cellular damage. We investigated the presence of an oxidative stress biomarker in saliva of patients after AMI. A prospective study was undertaken. AMI was characterized by laboratory (increased serum creatine phosphokinase (CPK) and troponin T), clinical (chest pain >; 20 minutes at rest) and electrocardiographic (changes in ST/T at least in two contiguous leads) criteria. At 24 and 48 hours, 2′7′ dichlorohydrofluorescein diacetate (DCFH‐DA) levels (spectrofluorimetry), a ROS index generation capacity, were assessed in saliva. Fifteen adult patients were admitted to our hospital with AMI and 12 healthy subjects, as controls. AMI patients showed serum CPK24>;CPK48 (t=3.29 p<0.006). In saliva, AMI group showed >; DCFH‐DA levels (t =3.295; p<0.003) that remained unchanged at 48 hours. In this preliminary study, AMI causes an increase of ROS which was detected in saliva by DCFH‐DA assay. Awarded by UBACyT 2011–2014, C001 and 20020100100613.