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Measurement of heart function for myocardial infarction model of rat by micro‐CT
Author(s) -
Matsushita Satoshi,
Naito Mayuko,
Amano Atsushi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1126.4
Subject(s) - medicine , cardiology , ejection fraction , myocardial infarction , infarction , pathological , contraction (grammar) , heart failure
Materials and Methods We demonstrated functional measurement with no (normal) and mild or severe myocardial infarction of the rat heart using micro‐computed tomography (μCT) with administration of contrast agent. The hearts were sectioned to assess pathological evaluation of the heart following with CT acquiring. Results The plot curve represented by each disk volume in normal heart revealed that contraction force was peaked at middle of the heart. In the mild infarcted heart, the volume plotting curve clearly demonstrated that infarcted area was limited at the apex of the heart, whereas that in severe infarction was in larger area. The left ventricular ejection fraction in normal, mild and severe was 68.6%, 40.0% and 16.4%, respectively. In addition, the volume analysis in severe infarction at seven days after injury demonstrated ventricular dilatation, which is consisted with histological evaluation. Conclusions Functional analysis of rat heart by μCT was accurate. This method is useful especially in small animal with heterogeneous dysfunction of the heart, since it can be analyzed with whole heart data.