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Ursolic acid potentiates muscarinic receptor‐induced ANP secretion in perfused beating rat atria
Author(s) -
Kim Hye Yoom,
Kwon Oh Jeong,
Kho Joung Hyun,
Tan Rui,
Cho Kyung Woo,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1126.11
Subject(s) - ursolic acid , muscarinic acetylcholine receptor , medicine , endocrinology , acetylcholine , chemistry , secretion , agonist , receptor , atrial natriuretic peptide , biology , chromatography
Ursolic acid has recently been reported to increase both atrial natriuretic peptide (ANP) secretion and mechanical dynamics in rabbit atria. The present study was designed to clarify the regulatory effects of ursolic acid on the ¥â‐adrenergic or muscarinic receptor‐mediated changes in ANP secretory and contractile function allowing measurement of atrial dynamics such as pulse pressure, stroke volume, and cAMP efflux in isolated perfused beating rabbit atria. Pretreatment with ursolic acid significantly attenuated the isoproterenol (¥â‐adrenergic agonist)‐ induced decrease in ANP secretion and increases in cAMP levels and atrial dynamics. Interestingly, ursolic acid concentration‐dependently accentuated the acetylcholine‐induced increase of ANP secretion and decrease of pulse pressure in the presence of isoproterenol (p < 0.001). These findings indicate that acetylcholine‐induced increase of ANP secretion is potentiated by ursolic acid, furthermore, acetylcholine‐induced decrease of atrial dynamics is also potentiated by ursolic acid, suggesting that ursolic acid regulates muscarinic receptor‐mediated secretory and contractile responses in perfused beating rabbit atria. Thus, this implicates for the beneficial effects of ursolic acid in the regulation of cardiovascular and body fluid homeostasis.