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Intrauterine undernutrition programs the hypothalamic proteome of female rats
Author(s) -
Ribeiro Eliane Beraldi,
Pedroso Amanda Paula,
Souza Adriana Pereira,
Dornellas Ana Paula Segantine,
Estadella Debora,
Nascimento Cláudia Maria Oller,
Oyama Lila Missae,
Rosa José Cesar,
Watanabe Regina LH
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1123.4
Subject(s) - proteome , energy homeostasis , malnutrition , endocrinology , medicine , biology , uterus , obesity , bioinformatics
Undernutrition in early life may cause adulthood obesity with disruption of energy homeostasis control. We analyzed the hypothalamic proteome of adult female rats born from dams fed ad libitum or restricted to 50% of ad libitum intake during gestation. Control and restricted progeny were studied at age 4 months. Hypothalamic proteins were analyzed by two‐dimensional gel electrophoresis and MALDI‐TOF/TOF–MS. MASCOT server was used to search Swiss‐Prot protein database. Only proteins with false discovery rate of 0% were considered in the results analysis. The restricted rats had normal body weight and insulin levels with increased fat pads weight and glycemia. Significant density increase of 10 spots and decrease of 9 spots was found. Six overexpressed and 4 under‐expressed proteins were identified. They are enzymes of energy metabolism pathways, and proteins of redox regulation, inflammatory, and cell communication processes. Female rats undernourished intra uterus developed into obese adults and presented alterations of hypothalamic protein profile that may be involved in the disturbances leading to obesity