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Promising applications of nano‐encapsulated (−) epigallocatechin gallate during adipogenesis in 3T3‐L1 cells
Author(s) -
Bapat Priyanka Vinayak,
Sun Ming,
Wang Shu
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.112.4
Subject(s) - adipogenesis , epigallocatechin gallate , triglyceride , chemistry , adipose tissue , white adipose tissue , endocrinology , medicine , biochemistry , antioxidant , biology , polyphenol , cholesterol
Background Overexpression of thermogenic genes in white adipose tissue can ameliorate obesity. Many studies reported acute thermogenic effects of epigallocatechin gallate (EGCG) in white adipocytes. However, its low stability limits its therapeutic applications. Objective of this study was to synthesize biocompatible nanoencapsulated EGCG (Nano‐EGCG) to increase stability and enhance biological effects. Methods Stability of Nano‐EGCG at 37°C was measured using HPLC system. 3T3‐L1 cells were treated at differentiation and maturation with native and Nano‐EGCG at 10 μM. Triglyceride content in mature adipocytes was measured by an enzymatic method. The gene expression levels were measured using real‐time PCR. Results Nano‐EGCG was more stable than native EGCG in vitro . Nano‐EGCG significantly (p<0.05) reduced triglyceride content in mature adipocytes as compared to EGCG. Moreover, when preadipocytes were induced to differentiate with Nano‐EGCG, the expression levels of peroxisome proliferator activated receptor gamma coactivator1‐alpha (PGC1α), and uncoupling protein family (UCP 1,2,3) were significantly increased as compared to native EGCG. Conclusion Nanoencapsulation significantly increased EGCG stability. Nano‐EGCG at low concentrations significantly reduced triglyceride content in mature adipocytes and increased the expression of thermogenic genes during differentiation. Grant Funding Source : TTU research incentive funding