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ALTERNATIVE PATHWAY TO ANGIOTENSIN CONVERTING ENZYME (ACE) FOR ANGIOTENSIN II GENERATION IN MOUSE MESENTERIC ARTERY
Author(s) -
Becari Christiane,
Barbosa da Silva Marcondes A.,
Salgado Helio C.,
Tostes Rita C. A.,
Oliveira Eduardo B.,
Salgado Maria Cristina O.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1119.3
Subject(s) - losartan , captopril , medicine , elastase , angiotensin ii receptor type 1 , endocrinology , angiotensin ii , chemistry , mesenteric arteries , angiotensin converting enzyme , superior mesenteric artery , renin–angiotensin system , receptor antagonist , ace inhibitor , antagonist , receptor , artery , enzyme , biochemistry , blood pressure
We investigated the Ang II forming enzymes in the mesenteric artery isolated from C57/BL6 mice. Cumulative concentration curves to Ang I and II were obtained in the absence or presence of losartan (AT1 receptor antagonist; 1 μM), captopril (ACE inhibitor; 10 μM) or chymostatin (serine proteases inhibitor; 100 μM), and association of captopril and chymostatin. Mesenteric arteries mRNA expression for ACE and elastase‐2 was obtained by real time‐PCR. Ang II and I produced a concentration‐dependent vasoconstrictor effect that was abolished by losartan. Ang II responses were not affected by the inhibitors. Chymostatin allowed a small but significant displacement of the curve to Ang I to the right (pPD 2 =7.3±0.3, p<0.05), while captopril induced greater rightward shift of the curve (pD 2 =5.4±0.3 vs 7.7±0.2 in controls, p<0.001). Captopril and chymostatin determined a further displacement of the curve to Ang I (pD2=4.4±0.4, p<0.05). mRNA for elastase‐2 and ACE were detected in mesenteric arteries. Ang II formation from Ang I is essentially dependent on ACE, although an alternative chymostatin‐sensitive pathway, probably elastase‐2, is also present in the mouse mesenteric artery. Supported by FAPESP and CNPq.