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Activation of NMDA receptors results in different autonomic and cardiovascular responses along the rostrocaudal axis of the insular cortex
Author(s) -
Marins Fernanda Ribeiro,
LimborçoFilho Marcelo,
Vaz Gisele Cristiane,
Xavier Carlos Henrique,
Fontes Marco Antonio Peliky
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1118.5
Subject(s) - nmda receptor , medicine , insular cortex , stimulation , receptor , endocrinology , cortex (anatomy) , antagonist , neuroscience , anesthesia , chemistry , psychology
Unilateral damage of the insular cortex (IC) causes imbalance of the autonomic control. The cardiovascular representation along the IC is unclear. We compared the cardiovascular responses evoked by stimulation of NMDA receptors into left (L‐IC) or right IC (R‐IC) in different levels of the IC rostrocaudal axis (−1.5, 0.0 and +1.5mm). Under urethane (1.4g/kg) anesthesia, Wistar rats were prepared for ECG, MAP, HR and RSNA recording. NMDA was injected unilaterally (0.2mM, 100nL; n=6–8/group). Gene expression of NMDA receptors in the IC was confirmed by RT‐PCR. Nanoinjection of NMDA into IC at −1.5 did not change the cardiovascular parameters. NMDA into IC at 0.0 increased RSNA (Δ: L‐IC 28±4 vs R‐IC31±5%) and HR (Δ: L‐IC 40±8 vs R‐IC 36±7 bpm) with small changes in MAP (Δ: L‐IC 5±1 vs R‐IC 7±2 mmHg). NMDA into IC at +1.5 decreased RSNA (Δ: L‐IC −24±4 vs R‐IC −19±2%), HR (Δ: L‐IC −35±4 vs R‐IC −40±5 bpm) and increased MAP (Δ: L‐IC 17±3 vs R‐IC 15±1 mmHg). NMDA evoked effects were blocked by its antagonist, AP‐5. Arrhythmias were not detected. We found different autonomic and cardiovascular responses in different levels of the IC rostrocaudal axis, but no differences between R‐IC and L‐IC. CNPq, Fapemig, Capes.