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Acute kidney injury following orthopedic trauma in obese Zucker rats
Author(s) -
Mittwede Peter,
Xiang Lusha,
Clemmer John,
Lu Silu,
Gowdey Andrew,
Hester Robert
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1114.6
Subject(s) - medicine , renal function , orthopedic surgery , acute kidney injury , kidney , basal (medicine) , excretion , renal blood flow , endocrinology , urine , urology , surgery , diabetes mellitus
An increased incidence of multiple organ failure occurs following trauma in obesity, but the extent and mechanism of exacerbated damage to specific organs is not understood. The kidney, in particular, is susceptible to organ failure. We hypothesized that there would be impairment in renal function following trauma in obesity. Lean (LZ) and obese (OZ) Zucker rats (11–13 wk, n=6 per group) were used. Orthopedic trauma was mimicked with bilateral hind limb soft tissue injury, followed by an injection of crushed bone components to the area. Twenty‐four hours after trauma, glomerular filtration rate (GFR), renal plasma flow (RPF), blood pressure (BP), and urine albumin measurements were taken. RPF was similar in all groups. LZ and OZ exhibited similar basal BP (122 ± 3 vs. 125 ± 5 mmHg, respectively) and GFR (1.51 ± .15 vs. 1.70 ± .13 ml/min/g). Trauma resulted in a significantly decreased BP (108 ± 3 mmHg) and GFR (0.89 ± .07 ml/min/g) in OZ with no changes in LZ (115 ± 3 mmHg, 1.40 ± .20 ml/min/g). As compared to pre‐trauma values, urine albumin excretion was significantly increased in the twenty hours following trauma in OZ (.63 ± .30 vs. 1.60 ± .51 mg), but not in LZ (.27 ± .05 vs. .51 ± .12 mg). These results show that in our model of orthopedic trauma, acute kidney injury occurs in obese but not in lean rats. This model will allow us to elucidate the mechanisms of trauma‐induced renal damage in obesity. Supported by NIH HL‐51971, HL‐89581, AHA‐12SDG12050525