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Sex does not impact asymmetric dimethylarginine (ADMA) or L‐arginine (L‐arg) levels in spontaneously hypertensive rats (SHR).
Author(s) -
Brinson Krystal N.,
Sharma Shruti,
Romero Maritza J.,
Sullivan Jennifer C.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1112.1
Subject(s) - medicine , endocrinology , asymmetric dimethylarginine , arginine , nitric oxide synthase , nitric oxide , chemistry , amino acid , biochemistry
ADMA and L‐arg are endogenous amino acids that impact nitric oxide synthase (NOS) activity. ADMA has been implicated in nitric oxide (NO) deficiency and hypertension. We have reported that female SHR have significantly greater NO bioavailability and NOS enzymatic activity in the renal inner medulla compared to age‐matched males, yet the mechanism responsible is unknown. We hypothesized that male SHR have greater ADMA levels and less L‐arg compared to females. ADMA and L‐arg levels in plasma (N=6–7) and renal inner medullas (N=10) were measured via HPLC from 12–13 week old male and female SHR. ADMA (Male: 20±6; Female: 31±8 nmols/ml, p=0.7), L‐arg (Male: 152±7; Female: 141±11 nmols/ml, p=0.7) levels and the L‐arg/ADMA ratio (p=0.2) in plasma were comparable between sexes. Renal inner medullary ADMA (Male: 172±55 Female: 192±58 grams wet weight (gww), p=0.8), L‐arg (Male: 507±107; Female: 552±127 gww, p=0.8) levels and L‐arg/ADMA ratio (p=0.8) were also comparable between groups. We conclude that while ADMA and L‐arg may be important in maintaining renal NOS activity in SHR, they do not contribute to sex differences in renal inner medullary NOS activity. Funded by the American Heart Association.