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Attenuated contractility in afferent arterioles during development of proteinuria in two‐kidney, one‐clip hypertensive rats
Author(s) -
Dahl Tone Dolva,
Skogstrand Trude,
Helle Frank,
Hultström Michael,
Tenstad Olav,
Iversen The late Bjarne Magnus
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1110.15
Subject(s) - proteinuria , kidney , endocrinology , medicine , afferent arterioles , renal cortex , contractility , blood pressure , chemistry , urine , angiotensin ii
Aims We wanted to investigate the contractile properties of afferent arterioles (AAs) from two‐kidney, one‐clip (2K1C) hypertensive rats before and during development of proteinuria, and whether these vessels behave differently in outer and inner renal cortex. Methods Wistar rats were subjected to either a 2K1C or sham operation. Blood pressure was measured and urine was sampled before the rats were killed after 18 ± 4 weeks. AAs were isolated from outer and inner renal cortex of the non‐clipped kidney of the 2K1C and from both kidneys of the sham rats with the agarose infusion technique. The AAs were stimulated with angiotensin II (AngII) in increasing concentrations (10 −10 M to 10 −7 M), and degree of contraction was recorded. Results The preliminary results are based on AAs isolated from 9 rats (Sham: 3, 2K1C without proteinuria ( 2K1C Prot−): 2, and 2K1C with proteinuria ( 2K1C Prot+): 4). There was no difference in mean arterial pressure between the 2K1C with and without proteinuria (184 ± 6 vs. 178 ±8 mmHg, NS). We found a significantly attenuated response to AngII 10 −7 M in the superficial AAs from both hypertensive groups compared to sham. However, in the AAs from the juxtamedullary nephrons there was a preserved response in the vessels from the 2K1C rats without proteinuria. Conclusion Loss of contractile properties of AAs in deep cortex may contribute to development of renal damage and proteinuria in this model.