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BXD mouse lines as a genetic reference population for metabolic syndrome
Author(s) -
LAMY Sebastien
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1109.9
Subject(s) - quantitative trait locus , type 2 diabetes , metabolic syndrome , obesity , biology , population , inbred strain , insulin resistance , gene , trait , genetics , diabetes mellitus , endocrinology , medicine , environmental health , computer science , programming language
Chronic multifactorial diseases as type 2 diabetes and obesity result from interaction of genes and environmental factors. In order to better understand this interaction, a mouse genetic reference population allowing control of both conditions was used in a pipeline of standardized metabolic tests (such as exercise endurance, adaptive thermogenesis, glucose tolerance test) in a diet‐induced obesity paradigm with chow diet (CD) groups versus high‐fat diet (HFD) groups over 29 weeks. A panel of 42 BXD recombinant inbred mouse lines, descending from C57BL/6J and DBA2/J strains, was used to identify quantitative trait loci (QTL) and gene networks that modulate metabolic activity. HFD groups showed a higher range in body weight increase than CD groups and significant differences in the BW loss after two weeks of voluntary exercise. Similarly, heterogeneity in the VO2 max, the cold resistance and the glucose tolerance is observed between strains and groups. After completion, the broad data set generated will enable QTL mapping strategies through the GeneNetwork resource. The outcome of this work will provide a better understanding of the metabolic syndrome with the identification of signaling pathways and network regulations and will initiate new strategies for diagnosis, prevention and therapy complex diseases associated with aging.