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Premium Bacterial stimulation of endogenous hydrogen sulfide synthesis: a novel mechanism for resolution and repair in the colon
Flannigan Kyle,
Verdu Elena,
Wallace John
Publication year2013
Publication title
the faseb journal
Resource typeJournals
PublisherThe Federation of American Societies for Experimental Biology
Hydrogen sulfide (H 2 S) is an important signaling molecule throughout the gastrointestinal (GI) tract. Colonic H 2 S synthesis is markedly increased during experimental colitis in rats, and contributes to the resolution of inflammation and healing of ulcers. The triggers responsible for this up‐regulation of colonic H 2 S synthesis are unclear. Therefore, we tested whether bacterial signals at the epithelial surface may be important stimuli for upregulation of endogenous H 2 S synthesis, helping drive resolution and mucosal repair. Germ‐free NIH Swiss mice (6 weeks of age; n≥4) were orally gavaged with feces (200 μL; 5% wt/ vol) from mice raised in specific pathogen free (SPF) conditions. Inoculated mice were housed in SPF conditions and euthanized at day 2 or 7 post‐inoculation. Colonic tissue from germ‐free control mice produced very low amounts of H 2 S. Within 2 days of inoculation with SPF flora, a significant increase in colonic H 2 S synthesis was observed (>;2‐fold; p<0.05), which further increased to 5‐fold (p<0.01) by 7 days post‐inoculation. We previously found that bacterial‐derived H 2 S does not contribute to what is measured as colonic H 2 S synthesis. These results support the hypothesis that bacterial factors can up‐regulate colonic H 2 S, which could be an important signaling mechanism for driving the repair of mucosal injury. Supported by the Crohn's and Colitis Foundation of Canada (CCFC).
Subject(s)biochemistry , biology , chemistry , colitis , downregulation and upregulation , endogeny , gastrointestinal tract , gene , hydrogen sulfide , immunology , medicine , microbiology and biotechnology , organic chemistry , pathogen , stimulation , sulfur
SCImago Journal Rank1.709

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