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Endothelial dysfunction accentuates the loss of function of beta adrenergic receptors in the smooth muscle of the rat aorta during maturation
Author(s) -
LopezCanales Oscar,
LopezCanales Jorge,
GuevaraBalcazar Gustavo,
CastilloHernandez Maria Carmen,
EscalanteAcosta Bruno
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1092.7
Subject(s) - adrenergic receptor , receptor , endothelium , medicine , endocrinology , period (music) , aorta , adrenergic , chemistry , function (biology) , biology , microbiology and biotechnology , physics , acoustics
It is well established that as age increases rat, the relaxing function of beta‐adrenergic receptors is lost gradually, however, the specific involvement of smooth muscle during this process is not yet well established. In an attempt to provide additional information on the topic, we evaluate the beta adrenergic relaxation response in an in vitro model, with two groups: rats of 3 and 9 weeks (maturation period) using arteries with and without endothelium. Our results indicate that during this period of maturation the relaxation obtained with isoproterenol decreases slightly (90 ± 3% vs 81 ± 2%) in aortic rings with endothelium, whereas the response in rings without endothelium the decrease is much larger (87 ± 4% vs 21 ± 2%). The next step was to try to establish that molecular changes smooth muscle suffers during this period, to this, there were concentration‐response curves in the presence of a wide range of inhibitors, and the results indicate that the signaling pathway used by these receptors does not change with age. In conclusion, the absence of endothelium increases the loss of function of beta adrenergic receptors, this effect cannot be explained by a change in the signaling pathway, which probably explained by changes in the expression and translation rate of the molecules involved.