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The oligomerized polyphenol oligonol ameliorates high glucose‐ and TNF‐α‐induced NADPH oxidase activation and insulin signaling down‐regulation in vascular endothelial cells
Author(s) -
Yokoo Hiroki,
Taguchi Kumiko,
Hattori Yuichi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1091.8
Subject(s) - nadph oxidase , endothelial dysfunction , oxidative stress , protein kinase b , downregulation and upregulation , medicine , oxidative phosphorylation , insulin receptor , chemistry , insulin , endocrinology , signal transduction , insulin resistance , biochemistry , gene
Endothelial dysfunction plays a key role in the pathogenesis of diabetic vascular disease. We investigated to explore the possible mechanism(s) by which the oligomerized polyphenol oligonol, used as an efficacious healthy aging dietary supplement, may be beneficial to endothelial cell dysfunction in diabetes. Porcine aortic endothelial cells were cultured in the presence of high glucose and TNF‐α with or without oligonol. In the presence of high glucose and TNF‐α, treatment of cells with oligonol prevented the increased expression level of the NADPH oxidase (Nox) subunit p67 Phox and the enhanced production of radical oxygen. Furthermore, the impaired phosphorylated levels of Akt and GSK‐3β and the down‐regulated expression level of insulin receptors were improved by oligonol treatment. These results indicate that oligonol can decrease the oxidative stress through inhibition of Nox activity and amend the insulin signaling pathway in the endothelial cells under conditions that mimic diabetes. This mechanism may offer an important standpoint for the beneficial cardiovascular health effects of oligonol.