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Develop a novel aptamer‐mediated assay for simultaneous detection of different types of circulating tumor cells in whole blood samples
Author(s) -
Zeng Zihua,
Parekh Parag,
Zhao Nianxi,
Zu Youli
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1088.18
Subject(s) - aptamer , circulating tumor cell , whole blood , chemistry , cancer cell , microbiology and biotechnology , fluorophore , fluorescence , oligonucleotide , antibody , cancer , biology , cancer research , biochemistry , dna , immunology , physics , quantum mechanics , genetics , metastasis
Oligonucleotide aptamers are a new class of small molecular probes. In contract to protein antibodies, aptamers can be chemically synthesized and easily modified with a variety of functional molecules. By conjugation of both fluorophore and quencher molecule we have previously developed a unique tumor cell‐activated aptamer reporter which is optically silent in the absence of cells of interest. However, the aptamer reporter can selectively target and highlight circulating tumor cells in whole blood with intracellular fluorescent signal. For simultaneous detection of different tumor cells, a new assay system containing aptamer reporters specific for multiple biomarkers (EpCAM, VEGF, and CD30) were formulated. Mixed tumor cells were added into the assay in a 96‐well microplate. After incubation for 30 minutes, the breast cancer cells, prostate cancer cells, and lymphoma cells were highlighted with different fluorescent signals, and simultaneously detected by fluorescent microscope without interference to each other. In addition, the new assay system was also validated in whole blood sample containing diluting cancer cells. Our study shows the first demonstration of a novel aptamer‐mediated assay, which is able to simultaneously detect different types of circulating tumor cells in a single assay with whole blood samples within minutes.