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Development of experimental protocol for photodynamic therapy on colorectal tumors: determination of irradiation by fluorescence spectroscopy
Author(s) -
Duarte Janaina,
Oliveira Ferreira Alexandre,
Junior Landulfo Silveira,
Plapler Helio
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1088.13
Subject(s) - photodynamic therapy , colorectal cancer , radiation therapy , medicine , fluorescence spectroscopy , prostate cancer , cancer , cancer research , fluorescence , chemistry , physics , organic chemistry , quantum mechanics
In the last decades, there was a significant improvement in traditional techniques such as surgery, chemotherapy and radiotherapy used to treat different types of neoplasias. Such therapies have intense side effects because they are not selective to the tumor tissue and these limitations and disadvantages have led to the search and development of new therapeutic alternatives to treat cancer, including photodynamic therapy (PDT) which, according to several studies conducted in the last two decades, have proved effective in malignant and pre‐malignant conditions, such as head and neck tumors, lung cancer, prostate cancer, brain tumors, duodenal and colorectal tumors. However, there is difficulty in determining the optimal protocol of PDT considering the main variables involved such as laser and photosensitive drugs. Kinects by fluorescence spectroscopy in tumors can be a useful tool to verify the ideal period of irradiation after photosensitive administration. This work developed a new protocol of kinects by fluorescence spectroscopy in colorectal tumor in Wistar rats to support the photodynamic therapy. For this protocol it was used chloroaluminum phthalocyanine tetrasulfonate and aluminum phthalocyanine chloride incorporated into liposomes as photosensitive drugs. The kinetics of both drugs was performed by means of fluorescence spectroscopy during 48h monitoring of chemically induced colorectal tumors in Wistar rats. The best irradiation period indicated by kinetics for both drugs was between 22 and 26 hours and the fluorescence spectroscopy has been showed useful to determine the PDT irradiation.