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Patterned laminin expression by spheroid‐forming melanoma cells
Author(s) -
Larson Allison R,
Lezcano Cecilia,
Lee ChungWei,
Huang John,
Zhan Qian,
Murphy George F
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1087.4
Subject(s) - vasculogenic mimicry , spheroid , laminin , melanoma , cell culture , in vitro , cancer research , immunohistochemistry , pathology , biology , chemistry , microbiology and biotechnology , medicine , cancer , metastasis , extracellular matrix , genetics
Vasculogenic mimicry, a collection of laminin networks within tumors is associated with a poor prognosis in melanoma and other cancers. Cultured melanoma cell lines that form spheroids are associated with increased invasiveness in vitro. Accordingly, we hypothesized that spheroid‐forming melanoma cells may also show patterned laminin networks. By immunohistochemistry, the A2058 melanoma cell line showed prominent patterned laminin networks within spheroids that closely mimicked networks that developed in primary melanoma xenografts to NSG mice. Real time PCR analysis of laminin subunits confirmed that anchorage‐independent spheroid‐forming cells expressed a different laminin profile from non‐spheroid‐forming adherent cells. These findings suggest that melanoma spheroids represent a novel approach to studies concerning the nature and significance of vasculogenic mimicry and associated laminin network formation. Funding provided by NIH Grant SPORE Core: NIH, NCI SPORE in Skin Cancer 5P50CA093683–08, BWH Biomedical Research Institute Microseed Grant, A.L. is supported by BWH Dept of Dermatology NIH Training Grant T32AR007098–38.