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Cav1 is a Key Mediator of Tumor‐Stromal Interactions in Melanoma
Author(s) -
Trimmer Casey,
Capozza Franco
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1087.16
Subject(s) - paracrine signalling , melanoma , stromal cell , tumor microenvironment , cancer research , metastasis , stroma , microbiology and biotechnology , biology , cytokine , chemistry , immunology , tumor cells , cancer , receptor , genetics , immunohistochemistry
Several lines of experimental evidence have demonstrated the importance of the tumor microenvironment in controlling melanoma tumor growth and melanoma metastasis. Cav1, the main structural component of the plasma membrane microdomains termed Caveolae, is emerging as an important signaling molecule in the stroma of several tumor types. However, Cav1's role in the melanoma microenvironment remains argely unexplored. Here, we utilize various experimental approaches to elucidate the function of stromal Cav1 in the development of melanoma in mice. We show that loss of Cav1 (but not Cav2) in mice promotes the growth of orthotopically implanted melanoma cells. We use cocultures of fibroblasts and melanoma cells coupled with cytokine analysis to identify various Cav1 regulated factors that function in a paracrine fashion to promote the growth of melanoma cells in vitro and in vivo . In summary, these studies reveal previously undefined functions for this protein in the melanoma microenvironment that could potentially be targeted for therapy.