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Effects of Dietary Protein and Vitamin A on Retinoids and Retinol binding Protein in Periparturient rats
Author(s) -
Foroudi Farhad,
Ramsey Kirk C.,
Rezamand Pedram
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1083.8
Subject(s) - retinol , medicine , endocrinology , retinoic acid , vitamin , retinoid , retinol binding protein , vitamin a deficiency , biology , tretinoin , chemistry , biochemistry , gene
Our objective was to study the effect of dietary vitamin A and protein on retinoid metabolism and retinoid binding protein (RBP) in vivo. Before the initiation of treatments (confirmed pregnancy), 4 rats were euthanized with blood and tissue samples obtained to establish baseline measures. Female rats, ~21 d before parturition, were fed rations altered in protein (13 or 22%) and vitamin A contents (3 or 5 IU/g). Samples were collected at d −14 (before parturition), d +3, and +10 postpartum via cardiac puncture under anesthesia (n=3–4/ group). Serum RBP showed no significant differences among treatments. Serum and liver 13‐cis retinoic acid (13‐cis), all‐trans retinoic acid (all‐trans) and retinol contents showed no differences, whereas serum all‐trans and retinol concentrations differed at −14 and +3 relative to baseline. The liver 13‐cis and all‐trans were significantly altered on d +3 and +10, and on d −14 relative to baseline, respectively. Treatments and time affected small intestine content of 13‐cis on d −14 and +3, and on d −14 and +10, respectively. The liver mRNA expression of RBP tended to differ for High Protein‐ High Vitamin group than other groups on d −14, whereas the liver TNF‐α gene expression tended to be lower for High Protein‐Low Vitamin group than other groups on d +3. Physiological state and dietary treatments affected the status of retinoids and RBP differently around the time of parturition.