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Impact of high saturated lipids diet on serum fatty acid profile in rats.
Author(s) -
Perris Paula Daniela,
Silva Carolina,
Fernandez Inés,
Mambrin Cecilia,
Slobodianik Nora,
Feliu María Susana
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1082.4
Subject(s) - weaning , chemistry , arachidonic acid , palmitic acid , oleic acid , fatty acid , linoleic acid , zoology , polyunsaturated fatty acid , medicine , biology , biochemistry , enzyme
Objetive to examine the effect of high saturated lipids diet on serum fatty acid profile. Weaning Wistar rats were fed with experimental diet containing 45kcal% fat provided by butter, complete in all nutrients. Group S1 received the diet for 10 days; S2 for 40 days; S3 received stock diet for 30 days and then 40 days of experimental diet. Control groups (C1, C2, C3) received normocaloric diet for the same time. Serum fatty acid profile was determined by gas chromatography. Results (area%±SD) were:S 1 C1 S 2 C 2 S 3 C 3Palmitic 18.10 ±6.48 17.84 ±2.12 20.63 ±2.35 * 16.08 ±2.15 17.25 ±2.15 * 16.44 ±1.62Oleic 19.12 ±5.18 * 10.60 ±2.01 20.37 ±2.06 * 11.29 ±2.27 17.71 ±2.85 * 10.08 ±1.34Linoleic 8.91± 1.79 * 19.99 ±4.68 9.79± 1.04 * 17.45 ±4.11 8.38± 0.79 * 17.82 ±3.38αLinolenic 0.49± 0.32 * 1.02± 0.46 0.44± 0.13 * 0.81± 0.22 0.43± 0.11 * 0.93± 0.18Arachidonic 6.36± 1.45 8.59± 2.15 10.77 ±1.05 * 16.32 ±4.27 17.52 ±2.30 * 22.78 ±1.98EPA 0.93± 0.82 0.83± 0.44 1.01± 0.15 1.13± 0.48 2.10± 0.20 1.83± 1.17DHA 1.22± 0.30 1.25± 0.24 2.76± 0.35 2.22± 0.75 1.76± 0.78 2.17± 1.10* p<0.01Experimental groups showed higher serum oleic acid levels with lower α‐linolenic and linoleic acids levels compared with control groups. Serum fatty acid levels changed in response to high fat% of experimental diet, the fatty acid profile, ‐which promotes ω9 pathway and decreases essential fatty acids‐, and to the time of administration. Supported by UBACyT 20020100200078