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Antidiabetic effects and antioxidant capacity of polyphenolenhanced Rutgers Scarlet Lettuce
Author(s) -
Cheng Diana Meimei,
Pogrebnyak Natasha,
Poulev Alexander,
Kuhn Peter,
Krueger Christian G.,
Waterman Carrie,
Raskin Ilya
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.7
Subject(s) - polyphenol , chlorogenic acid , antioxidant , chemistry , food science , anthocyanin , flavonols , quercetin , in vivo , biochemistry , biology , microbiology and biotechnology
Many health‐promoting properties of botanicals are associated with their high polyphenol content and their strong antioxidant activity. To produce a polyphenol‐enhanced botanical, anthocyanin‐rich leaves were selected by tissue culture to develop Rutgers Scarlet Lettuce (RSL). RSL contained greater levels of polyphenolic compounds (~94 mg/g dry weight of leaves), including anthocyanins and flavonols, compared to their parental varieties. RSL also showed greater antioxidant capacity compared to parental varieties as well as commercial lettuce and blueberries. Three major phytochemicals associated with antidiabetic and/or antiinflammatory activities accumulated in RSL: chlorogenic acid (~26 mg/g), cyanidin‐malonyl‐glucoside (~19 mg/g), and quercetin‐malonyl‐glucoside (~34 mg/g). RSL also demonstrated anti‐diabetic effects in vivo and in vitro. In high fat diet‐induced obese mice, daily oral administration of lyophilized RSL leaves (300 mg/kg) for 6–8 days significantly lowered fasting blood glucose levels compared to vehicle (water) and improved insulin mediated glucose metabolism. Aqueous extracts of RSL and purified chlorogenic acid showed dose‐dependent inhibition of glucose production in H4IIE rat hepatomas. These results suggest daily consumption of polyphenol‐rich RSL may provide beneficial health effects and have the potential to ameliorate diabetic conditions. Grant Funding Source : NIH/NCCAM/ODS P50AT002776–01. NIH training grant T32: 5T32AT004094–04