Premium
Suppression of TPA‐induced Invasion of HepG2 cells by Glyceollins
Author(s) -
Kim JongSang,
Park In Sil,
Kim Hyo Jung,
Kim Sung Soon,
Kang Hye Ryung,
Kim Bo Ram,
Zakaroba Alexandra,
Seo Ji Yeon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.57
Subject(s) - protein kinase b , blot , chemistry , metastasis , cancer research , matrix metalloproteinase , cancer cell , kinase , isoflavones , medicine , cancer , endocrinology , phosphorylation , biology , biochemistry , gene
Our previous study demonstrated that glyceollins (mixture of isomers I, II, and III), prenylated isoflavones derived from daizein in soybean elicited by fungi, had cancer preventive effect and antitumor activity. However, it is not known whether glyceollins inhibit the invasion of cancer cells. The purpose of this study was to assess the effect of glyceollins on the invasion of human hepatoma cells (HepG2) by 12‐O‐tetradecanoylphorbol 13‐acetate (TPA). Effects of glyceollins on cancer cell invasion were evaluated using wound healing and transwell assays. The effect of glyceollins on the expression of matrix metalloproteinase (MMP)‐ 9, mitogen‐activated protein kinases (MAPKs), AKT, and nuclear factor‐kappa B (NF‐kappa B), was investigated with gelatin zymography and western blotting. Glyceollins significantly suppressed TPA‐induced cell invasion in a dose dependent manner, which was accompanied with suppression of TPA‐induced MMP‐9 expression and activation of NF‐kappa B. Taken together, our results suggest that glyceollins inhibit the metastasis of human hepatoma cells by suppressing the expression of MMP‐9 which is mediated by NF‐kappa B signaling pathway.