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Biological activities of water‐soluble sulfated polysaccharides from Ecklonia cava , Enteromorpha prolifera and Monostroma nitidum
Author(s) -
Hien Hoang Minh,
Kim JiYoung,
Lee SungJoon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.54
Subject(s) - antioxidant , chemistry , polysaccharide , sulfation , biochemistry
Water‐soluble sulfated polysaccharide (WSSP) extracted from Ecklonia cava , Enteromorpha prolifera and Monostroma nitidum and fractionated using ion‐exchange chromatography (F0, F1, F2 and F3 fraction) were investigated for their antioxidant, hypolipidemic and anti‐inflammatory in vitro activities. We found that the WSSP fractions, especially the ECF1 and ECF2 in Ecklonia cava , EF1 in Enteromorpha prolifera and MF1 and MF3 in Monostroma nitidum , exhibited strong antioxidant activity as assessed by superoxide dismutase assay. The stimulation of lipid‐loaded HepG2 cells with the ECF1, ECF2, EF1, MF1, and MF3 significantly reduced cellular lipid concentrations as well. Quantitative PCR analysis revealed that the decrease in cellular lipid levels were due to reducing cholesterol synthesis gene expression and increasing mRNA levels in cholesterol degradation, LDL uptake, and peroxisomal β‐oxidation. In addition, expressions of genes related to inflammation including iNOS, TNF‐γ, IL‐6, IL‐ 8 and Visfatin were suppressed in lipid‐loading hepatocytes stimulated with the ECF 1, ECF2, EF1, MF1, and MF3 fractions. Together, these results suggest that the water‐soluble sulfated polysaccharides of Ecklonia cava , Enteromorpha prolifera and Monostroma nitidum could be considered as a potential antioxidant, hypolipidemic and anti‐inflammatory agent.