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The natural carotenoid astaxanthin, a PPAR modulator, ameliorates hepatic steatosis in C57BL/6 mice
Author(s) -
Jia Yaoyao,
Lee SungJoon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.46
Subject(s) - steatosis , endocrinology , astaxanthin , medicine , fatty liver , lipid metabolism , in vivo , chemistry , triglyceride , hepatocyte , lipid droplet , carotenoid , biology , in vitro , biochemistry , cholesterol , microbiology and biotechnology , disease
Astaxanthin (AX), a natural carotenoid abundant in seafood, has hypolipidemic activity. Previously, we reported that AX is a PPARα agonist and PPARγ antagonist in reporter gene assay and time‐resolved fluorescence energy transfer analyses, via direct binding to PPAR subtypes measured by surface plasmon resonance experiments, and significantly reduced cellular lipid accumulation in lipid loaded hepatocytes. To confirm the effects of AX in vivo , we fed C57BL/6 mice by standard and high fat diet (HFD) for 8 weeks to induce the hepatic steatosis, and then orally administrated with olive oil with (6 mg/kg body weight and 30 mg/kg body weight) or without AX for 8 weeks. AX feeding significantly improved liver histology of HFD fed mice and reduced the level of triglyceride both in plasma and in liver. Although AX administration has not changed the body and liver weight of the mice, the abdominal fat weight was significantly lower in high dosage of AX fed mice compared with the HFD fed mice. We concluded that AX reduces hepatic lipid accumulation in lipid‐loaded hepatocytes and HFD fed mice, and the effects of AX may via regulate the lipid metabolism in the hepatocytes and liver by the net effects of activating PPARα and inhibiting PPARγ simultaneously.