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Acute toxicity of lotus ( Nelumbo Nucifera ) seed ethanol extract in ICR mice
Author(s) -
You Jeong Soon,
Kim Sung Hoon,
Lee Yun Ju,
Kim So Young,
Zhao Xu,
Chang Kyung Ja
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.43
Subject(s) - lotus , nelumbo nucifera , toxicity , body weight , acute toxicity , traditional medicine , median lethal dose , spleen , kidney , biology , physiology , medicine , zoology , toxicology , botany , endocrinology
Lotus ( Nelumbo Nucifera ) is widely distributed in Korea, China, India and Japan area and lotus seed has been used as a folk medicine. The purpose of this study was to evaluate acute toxicity information of lotus seed ethanol extract (LSEE) in ICR mice. Twelve mice of each sex were randomly assigned to 3 groups of 4 mice each and were administered singly by gavages at doses of 0, 2000 and 5000 mg LSEE/kg body weight. After single oral administration, sings of toxicity such as behavioral changes and mortality were observed every hour for the first 6 hours and every day for 14 days. At the end of the 14th day experiment period, all mice were sacrificed. There was no difference in body weight change, diet intake, water consumption and organs weights such as liver, kidney, lung and spleen among different dose groups. At doses of 2000 and 5000 mg LSEE/kg body weight, mortality, macroscopic changes of internal organs and abnormal behavioral changes were not observed. The results showed that LSEE did not produce any signs of toxicity and the LD 50 and approximate LD of LSEE in both female and male ICR mice were considered as over 5,000 mg/kg body weight. This research was supported by the Globalization of Korean Foods R&D program, funded by MIFAFF, Republic of Korea