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Analysis of Coenzyme Q10 in Marine Oil Supplements
Author(s) -
Scholl Chris,
Dever Joseph,
Barnes David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.23
Subject(s) - coenzyme q10 , saponification , fish oil , marine fish , chemistry , food science , vegetable oil , high performance liquid chromatography , chromatography , fish <actinopterygii> , biology , fishery , biochemistry
CoQ10 supplementation has been promoted to prevent and/or reduce symptoms of several degenerative diseases including arteriosclerosis and hypertension. Natural sources of CoQ10 include certain vegetable oils, meats, nuts, and fish. Marine oils derived from cod liver, tuna and calamari were investigated as unique natural sources of CoQ10; however, analysis was problematic due to the complex sample matrix. After unsuccessful attempts measuring CoQ10 using a method designed for vegetable oils, a HPLC‐UV method was developed to determine CoQ10 in marine oils. Sample preparation included saponification followed by solvent extraction. Of the seven commercially available marine oil supplements measured calamari oil had the highest levels of CoQ10 (>;200 mg/kg).These results were confirmed using a separately developed quantitative LC‐MS (Q‐TOF) method which utilized isotopically labeled CoQ10 as an internal standard. Our results demonstrate that a 5mL serving of calamari oil provides approximately 20% of the average daily intake of CoQ10.