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Isolation of anti‐obesity active compound orientin from Lythrum salicaria L.
Author(s) -
Kim Hee yeon,
Lee Ki yeon,
Hong Suyoung,
Lim Sang Hyun,
Kim Kyung Hee,
Kim Songmun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1079.16
Subject(s) - orientin , butanol , ethyl acetate , chemistry , dichloromethane , ethanol , lipase , hexane , chromatography , methanol , traditional medicine , solvent , organic chemistry , enzyme , flavonoid , vitexin , medicine , antioxidant
The objective of this experiment was to isolate anti‐obesity active compound from Lythrum salicaria L. and to identify its chemistry. The ethanol extract of Lythrum salicaria L. was sequentially fractionated with n‐hexane, dichloromethane, ethyl acetate, butanol and H2O. Butanol fraction inhibited lipase activity. BD fractions from butanol were obtained by eluting butanol extracts with a mixture solution of water‐acetonitrile‐methanol in a ODS gel column chromatography. The BDC fraction showed strong inhibitory effect against lipase activity. The chemical structure of the BDC fraction was confirmed as orientin by analysis of MS, 13C‐NMR, 1H‐NMR. Overall results of this study indicated that the leaf extracts of Lythrum salicaria L. have anti‐obesity activity and such extracts could be used for the development of health functional foods.