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Paternal folate deficiency influences hepatic DNA methylation, IGF‐1, IGF‐2 and IGF‐1R expression in the rat pup
Author(s) -
Mejos Karen Kay Rosario,
Lim Eun Mi,
Kim Hye Won,
Chang Namsoo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1077.27
Subject(s) - medicine , endocrinology , methylation , dna methylation , homocysteine , lactation , insulin like growth factor , biology , folic acid , growth factor , receptor , gene expression , pregnancy , dna , gene , biochemistry , genetics
We investigated the effect of paternal folate deficiency on the hepatic folate content, DNA methylation, insulin‐like growth factors 1 and 2 (IGF‐1 and IGF‐2) and IGF‐1 receptor (IGF‐1R) expressions, as well as serum homocysteine (Hcy) level in the 3‐ week‐old pups. After 4 weeks of intervention with folic acid‐deficient (D) or folic acid‐supplemented (S) diet, paternal (P) and maternal (M) rats were mated and grouped as PSxMS, PSxMD, PDxMS, and PDxMD. Pups were sacrificed at day 21 of lactation. Folate content, DNA methylation and IGF expressions were analyzed in the liver, while Hcy level was analyzed in the serum of the pups. Liver folate content was markedly reduced in PDxMS group, while PSxMD group decreased as much as the level of PDxMD group (P<0.0001). Serum Hcy levels were elevated in PSxMD and PDxMD groups (P<0.0001). Hepatic DNA methylation was decreased in PSxMD and PDxMS groups as much as that in PDxMD group (P<0.0001). Hepatic IGF‐1, IGF‐2 and IGF‐1R expressions were downregulated in PDxMD and PDxMS groups, followed by PDxMS group, as compared to PSxMS group (P<0.05; P<0.01; P<0.01). These data indicate that paternal folate deficiency at mating, despite adequate maternal folate status, can influence hepatic folate content, global DNA methylation, as well as IGF‐1, IGF‐2 and IGF‐1R expressions in the pup. This research was supported by the BSRP through the NRF of Korea (2011–0003072), and BK21.