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2‐Hydrazinoquinoline as a novel derivatization agent for LC‐MS‐Based metabolomic investigation of streptozotocin‐elicited ketoacidosis
Author(s) -
Lu Yuwei,
Yao Dan,
Chen Chi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1073.3
Subject(s) - derivatization , chemistry , aldehyde , streptozotocin , ketone , carboxylic acid , ketone bodies , metabolomics , metabolic pathway , ketoacidosis , diabetic ketoacidosis , metabolism , chromatography , mass spectrometry , organic chemistry , biochemistry , diabetes mellitus , endocrinology , catalysis , type 1 diabetes , medicine
Aldehydes, ketones, and carboxylic acids are important intermediates and end products of many metabolic processes. In this study, the use of 2‐hydrazinoquinoline (2‐HQ) as a novel derivatization agent for LC‐MS analysis of aldehydes, ketones, and carboxylic acids in biological samples was explored, and the conditions for the derivatization reaction were optimized. The formation of carboxylic acid derivatives is attributed to the esterification reaction between 2‐HQ and carboxyl group, while the production of aldehyde and ketone derivatives is through the formation of Schiff bases between 2‐HQ and carbonyl group. Using this 2‐HQ‐based approach, the metabolic disorder induced by streptozotocin‐elicited diabetes was examined by the LC‐MS‐based metabolomics. The results showed the time‐dependent separation of mouse urine samples from STZ treatment in a multivariate model of urinary metabolites. Both known and novel small‐molecule biomarkers associated with STZ‐induced ketoacidosis were conveniently identified and subsequently elucidated, reflecting the dramatic changes in nutrient (glucose, amino acid, and lipid) and energy metabolism after STZ treatment.