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Vitamin E and Vitamin C supplementation does not prevent glucose intolerance in obese‐prone rats
Author(s) -
Picklo Matthew J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1067.3
Subject(s) - medicine , endocrinology , calorie , obesity , vitamin e , vitamin , vitamin c , chemistry , oxidative stress , antioxidant , biochemistry
Obesity‐induced glucose intolerance affects over 70 million Americans. Elevated oxidative stress is associated with development of glucose intolerance. In this work, we tested the hypothesis that supplementation with the anti‐oxidants vitamin E (d‐alpha‐tocopherol acetate; 0.4 g/kg diet) and vitamin C (0.5 g/kg diet) prevents glucose intolerance in obese‐prone Sprague‐Dawley (OBSD) rats. OBSD rats were fed either a low‐fat diet (LF; 10% of calories from lard; n=14), a high‐fat diet (HF, 45% of calories from lard; n=14), or a high‐fat diet with supplemented vitamin E and vitamin C (HF‐CE; n=14). Induction of obesity was characterized by significantly elevated body fat and hyperlipidemia. Anti‐oxidant supplementation had no effect upon these parameters. Following 16 weeks on this diet, oral glucose tolerance tests were performed with a dose of glucose 1.4 g/kg per kg lean body mass. While the HF diet caused glucose intolerance, anti‐oxidant supplementation did not prevent obesity‐induced increases in fasting glucose levels, HF: 75± 6 mg/dL, HF‐CE:76 ± 5 mg/dL, and LF:64±8 mg/dL nor prevented obesity‐induced increases in the glucose area under the curve: HF: 13,155 ± 1516 mg*min/dL, HF‐CE: 13211 ± 1063 mg*min/dL, and LF 10411 ± 1328 mg*min/dL. These data indicate that vitamin E and vitamin C supplementation does not prevent glucose intolerance. This work was supported by the USDA‐ARS.