Premium
Osteogenic activity of milk thistle extract after ovariecotmy to dampen estrogen deficiency‐induced osteoporosis
Author(s) -
Kim JungLye,
Kim Yunho,
Lee EunJung,
Kang YoungHee
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1053.11
Subject(s) - ovariectomized rat , endocrinology , medicine , bone resorption , osteoporosis , osteopenia , osteoclast , bone remodeling , chemistry , bone mineral , osteoprotegerin , acid phosphatase , resorption , cathepsin k , estrogen , activator (genetics) , receptor , biochemistry , enzyme
Bone integrity abnormality and imbalance between bone formation by osteoblasts and bone resorption by osteoclasts results in metabolic bone diseases such as osteoporosis. This study evaluated the suppressive effects of milk thistle extract (MTE) on bone loss. C57BL/6 female mice were ovariectomized (OVX) as a model for postmenopausal osteopenia and administered MTE (10 mg/kg per day) or MTE component silibinin orally for 8 weeks. The sham‐operated mice served as controls. MTE and silibinin enhanced alkaline phosphatase activity of osteoblasts but reduced tartrate resistant acid phosphatase (TRAP) activity of osteoclasts. The osteoprotective effects of MTE were comparable to those of estrogenic isoflavone. Low‐dose combination of MTE and isoflavone had a pharmacological synergy that may be useful for osteogenic activity. The treatment with nontoxic MTE and silibinin in ovariectomized mice improved femoral bone mineral density and serum receptor activator of nuclear factor‐κB ligand/osteoprotegerin ratio, an index of osteoclastogenic stimulus. In addition, MTE administration inhibited bone loss of the femur induced by ovariectomy and induction of cathepsin K necessary for osteoclast activitybone resorption. Collectively, oral doses of MTE in the preclinical setting are effective in preventing estrogen deficiency‐induced bone loss.