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Apigenin and Kaempferol inhibit LPS‐induced inflammatory responses by regulating intracellular translocation of RNA‐binding protein HuR in macrophages
Author(s) -
Zhang Xiaoxuan,
Wang Guangji,
Liu Runping,
Zhang Luyong,
Hylemon Phillip B,
Pandak William M,
Zhou Huiping
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1033.2
Subject(s) - apigenin , kaempferol , western blot , proinflammatory cytokine , tumor necrosis factor alpha , inflammation , intracellular , chromosomal translocation , chemistry , biology , microbiology and biotechnology , biochemistry , immunology , flavonoid , gene , antioxidant
Inflammation is a key player in numerous human diseases including metabolic diseases and cardiovascular diseases. Natural flavonoids are the major components of traditional Chinese medicine and have been used to treat various inflammatory diseases. However, the underlying cellular/molecular mechanisms remain unknown. Apigenin and kaempferol are major, naturally‐occurring plant flavonoids and abundantly present in common fruits and vegetables. The aim of this study was to examine the effect of apigenin and kaempferol on LPS‐induced inflammatory response and further identify the underlying cellular/molecular mechanisms in macrophages. Methods Cultured mouse J774A.1 macrophages and human THP‐1‐derived macrophages were used in this study. The mRNA and protein levels of IL‐1β, IL‐6, TNF‐α, MCP‐1, and COX‐2 were detected by real time RT‐PCR, Western blot and ELISA, respectively. The intracellular distribution of RNA binding protein HuR was determined by Western blot and immunofluorescence staining. Results Both Apigenin and Kaempferol significantly inhibited LPS‐induced expression of proinflammatory cytokines (IL‐1β, IL‐6, TNF‐α, MCP‐1) and COX‐2. LPS‐mediated cytoplasmic translocation of HuR was inhibited by apigenin and Kaempferol. Conclusion Apigenin and Kaempferol inhibit LPS‐induced inflammatory responses through regulating intracellular translocation of HuR in macrophages.