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Characterization of the role of long chain acyl‐CoA synthetase 6 in skeletal muscle energy metabolism
Author(s) -
Bu So Young
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1017.13
Subject(s) - skeletal muscle , glut4 , gene knockdown , c2c12 , myocyte , myogenesis , lipid metabolism , carbohydrate metabolism , biology , metabolism , glucose uptake , biochemistry , microbiology and biotechnology , endocrinology , insulin , gene
Previous studies suggest that numerous isoforms of long chain acyl‐CoA synthetase (ACSL) mediate intracellular fatty acids channeling and regulate energy metabolism. However the role of these genes in muscle need to be identified in order to understand how they contribute to whole body energy metabolism. This study was designed to characterize how ACSL6 is regulated and the function of this gene in skeletal muscle energy metabolism. C2C12 mouse myoblasts were utilized and expression of genes that involving glucose and fatty acids metabolism was measured during cellular differentiation. To characterize the role of ACSL6 in skeletal muscle energy metabolism, siRNA mediated knockdown of ACSL6 was performed. During myogenic differentiation gene expressions involving glucose and lipid metabolism including GLUT4 and ACSL6 were increased while they were not or barely detected in pre‐confluenced myoblasts. Compared to cells transfected with control siRNA, knockdown of ACSL6 significantly decreased GLUT4 expression. These results suggest that lipid channeling system is developed in the course of myogenic differentiation, and intracellular fatty acids trafficking derived from ACSL6 is partially responsible for glucose utilization in skeletal muscle cells. Additional studies how ACSL6 modulate glucose utilization and insulin signaling in skeletal muscle cells remain to be performed. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded b y the Ministry of Education, Science and Technology (2012R1A1A1019253).