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Mammalian Ste20‐like protein kinase 3 mediates the bioenergetics of mitochondria
Author(s) -
Yuan ChiunJye,
Chen ChihYen
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1011.6
Subject(s) - microbiology and biotechnology , gene knockdown , cytoplasm , mitochondrion , hela , biology , bioenergetics , kinase , protein kinase a , clone (java method) , cell , apoptosis , biochemistry , gene
Mst3 (mammalian Ste20‐like serine/threonine protein kinase 3), a key participant in the stress‐ and drug‐induced apoptosis, was recently shown recently to be present both in cytoplasm and in intermembrane space of mitochondria in different cell lines. These results suggest that the mitochondrial localization of Mst3 is a general phenomenon. Interestingly, the selective knockdown of Mst3 by RNA interference resulted in a significant reduction of the protein level of respiratory transport chain complexes I and III. Further studies showed that HeLa stable clone with selective knockdown of Mst3, HeLa(siMst3), exhibited an increased dependence of glucose for ATP synthesis. Compared with controls, the HeLa(siMst3) stable clone was less viable in a glucose‐depriving cultural medium. The mitochondrial‐targeted recombinant Mst3 and its mutants were constructed and expressed in the cells to demonstrate that Mst3 play a role in controlling the bioenergetics of mitochondria. These results indicate that the biological functions of Mst3 are spatially controlled in cells.