Premium
Using Long‐Extension PCR Assay to Detect Large‐Scale Deletions in the Mitochondrial DNA
Author(s) -
Pogozelski Wendy Knapp,
Chiappone Sam,
Evans Christian,
O'Donnell Robert
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1011.10
Subject(s) - mitochondrial dna , amplicon , biology , dna , microbiology and biotechnology , genetics , human mitochondrial genetics , genome , computational biology , ionizing radiation , polymerase chain reaction , gene , physics , irradiation , nuclear physics
Human mitochondrial DNA (mtDNA) is subject to large‐scale deletions. These deletions occur at low levels and are difficult to detect. We show efforts to develop a PCR assay to amplify large sections of the mitochondrial genome, compare the lengths of the amplicons and thus detect the presence of deletions. We also show this technique can be used to determine the effect of insults such as ionizing radiation.