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Metabolic responses of the aerobic anoxygenic phototrophic bacterium Roseobacter denitrificans during photoheterotrophic and heterotrophic growth
Author(s) -
Tang Joseph KuoHsiang
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1008.1
Subject(s) - roseobacter , anoxygenic photosynthesis , heterotroph , phototroph , biology , biomass (ecology) , botany , bacteria , food science , chemistry , biochemistry , photosynthesis , ecology , clade , genetics , gene , phylogenetic tree
Roseobacter denitrifican is one of the first two reported species in Roseobacter clade members, which comprise up to 25% of total marine bacterial community and play essential roles on global carbon cycle. Here, we reported the studies of acetate metabolism of Rsb. denitrificans grown under light, dark and light‐dark cycles by investigating transcriptome dynamics, cell dry weight, pigment production and other physiological changes. Acetate‐grown Rsb. denitrificans produce more pigments and less biomass during heterotrophic growth than during photoheterotrophic growth (Fig. 1). Moreover, while Rsb. denitrificans has been reported to be an obligated photoheterotroph, our studies suggest that the bacterium employs the 3‐hydroxypropionate (3HOP) bi‐cycle for assimilating carbon when growing on acetate and bicarbonate. The 3HOP bicycle uses HCO 3 − as the inorganic carbon source that does not compete with photorespiration. Further, biofilm was found for acetate/bicarbonate‐grown cultures, but not acetate‐grown cultures, with more being produced during phototrophic growth than during heterotrophic growth.