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Up‐Regulation of Replication Factor C‐40 (RFC40) in estrogen positive as well as negative breast cancer
Author(s) -
Gupte Rakhee
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb86
Subject(s) - breast cancer , polysomy , cancer research , biology , cancer , estrogen receptor , fluorescence in situ hybridization , microbiology and biotechnology , gene , genetics , chromosome
Background Currently most therapies for breast cancer are targeted against growth factor and endocrine receptors. Identification of non receptor based molecules that will target receptor negative breast cancers is of paramount importance. Replication Factor C 40 (RFC40) protein required for DNA replication and it influences the cell cycle from DNA replication to cell division. RFC40 is over‐expressed in choriocarcinoma, acute and chronic myeloid leukemia, nasopharyngeal cancer and glioblastomas, suggesting that it may be directly responsible for unrestricted DNA replication and cell proliferation. However, its role in breast cancer has not been explored. Methods We determined the expression of RFC40 message and protein by qRT‐PCR and Western blot analyses in normal, estrogen positive and negative breast cancer cell lines and performed immunohistochemistry for RFC40 protein in patient breast tumor microarrays (BTMAs). We performed fluorescent in situ hybridization analyses with BTMAs to determine amplification of the RFC40 gene copy numbers in breast cancer. Results We found that RFC40 message and protein is significantly up‐regulated in all the breast cancer cell lines and in the patient BTMAs. We observed RFC40 gene amplification and polysomy of chromosome 7 in BTMAs Conclusions RFC40 is up‐regulated in breast cancer accompanied by either gene amplification or polysomy of chromosome 7.