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Protective Effect of Hypercapnia on Ischemia – Reperfusion Injury in Lungs Retrieved from Non‐heart‐beating Donors
Author(s) -
Hodyc Daniel,
Suva Petr,
Mrazkova Hana,
Skoumalova Alice,
Hampl Vaclav,
Herget Jan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb821
Subject(s) - hypercapnia , medicine , anesthesia , ischemia , lung , ventilation (architecture) , lung transplantation , reperfusion injury , warm ischemia , transplantation , acidosis , cardiology , mechanical engineering , engineering
Lungs retrieved from non‐heart‐beating donors (NHBD) might alleviate the shortage of suitable organs for transplantation. Since hypercapnia has protective effect on free radical damage, in present study we investigated a possible protective effect of hypercapnic ventilation during reperfusion in lungs retrieved from NHBD. Experimental rats underwent the protocol of NHBD lung harvesting: 90 minutes of warm ischemia after pentobarbital euthanasia followed by 60 min of cold ischemia (12°C). Then we measured perfusion pressure, lung weight gain and arterio ‐ venous difference in O2 partial pressure in isolated, Salt solution + Ficol perfused lungs. In controls, lungs were harvested immediately after euthanasia. We used four groups of rats: Control group – C Control group, isolated lungs ventilated with hypercapnic gas (10%CO2) ‐ CH Experimental rats – warm + cold ischemia – W Experimental rats, hypercapnic ventilation – WHWarm ischemia deteriorates lung functions (W vs C). We found that hypercapnic ventilation reduced pulmonary edema (WH vs. W) and improved oxygen transport ability (WH vs. W, no difference in WH vs. C). We conclude that hypercapnia prevents ischemia – reperfusion injury in NHBD lungs. Supported by Czech Science Foundation, GACR P303/10/P343 and GACR 305/08/0108