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Early‐stage changes to neurovascular morphology and telemetric measurement of sympathetic nerve activity in streptozotocin‐induced diabetic rats
Author(s) -
Grise Kenneth Neil,
Hall Katharine E,
McDonald Matthew W,
Noble Earl G,
Melling C.W. James
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb802
Subject(s) - medicine , neurovascular bundle , diabetes mellitus , streptozotocin , sciatic nerve , endocrinology , cardiology , anesthesia , anatomy
Chronic sympathetic nerve overactivity is an established consequent of Type 1 Diabetes Mellitus (T1DM) and is known to induce both micro and macroangiopathy. Telemetry is used to monitor real‐time sympathetic nerve activity (SNA) to measure neuropathic progression in T1DM. This study aimed to examine changes in the neurovascular morphology of early‐stage streptozotocin (STZ)‐induced T1DM rats and determine if telemetry is sensitive to neuropathic changes in SNA. Animals were separated into control (C, n=9) and T1DM (D, n=9) groups. Diabetes was induced using multiple doses of STZ consisting of 20mg/Kg per day for 5 days. In three animals from each group, electrodes were placed around isolated renal nerves and telemeters were implanted abdominally. Measurements of SNA were recorded weekly over 12 hour periods. All animals were euthanized 6 weeks post STZ treatment. Compared to C, D showed a decrease in blood vessel number ( t (10) = 4.44, p < 0.001) and size ( t (10) = 3.60, p < 0.05), an increase in sympathetic fiber size ( t (9) = –2.34, p < 0.05) but no difference in fiber number in sciatic nerves. Despite these morphological changes, SNA in C and D was not statistically different in frequency or amplitude at any time‐point during the 6 week period. Though morphological changes are observed early in STZ diabetes, neurophysiological changes recorded by telemetry may be too slight or variable to be clearly distinguished.